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Definition of Relative fluorescence unit (rfu)

Relative fluorescence unit (rfu) Definition from Law Dictionaries & Glossaries
President's DNA Initiative Glossary
a unit of measurement used in electrophoreses methods employing fluorescence detection. Fluorescence is detected on the CCD array as the labeled fragments, separated in the capillary by electrophoresis, and excited by the laser, pass the detection window. The software interprets the results, calculating the size or quantity of the fragments from the fluorescence intensity at each data point.
  
Source: The President's DNA Initiative ( About )