Affinity chromatography
A technique in which a biospecific adsorbent is prepared by coupling a specific ligand (such as an enzyme, antigen. or hormone) for the macromolecule of interest to a solid support (or carrier). This immobilized ligand will interact only with molecules that can selectively bind to it. Molecules that will not bind elute unretained. The retained compound can later be released in a purified state. Affinity chromatography is not a chromatographic technique as such, but is actually selective filtration. | ||||
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Affinity chromatography Definition from Encyclopedia Dictionaries & Glossaries
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Affinity chromatography
Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Affinity chromatography combines the size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules.
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